Journal: NPJ Vaccines

Article Title: A Lassa virus live attenuated vaccine candidate that is safe and efficacious in guinea pigs

doi: 10.1038/s41541-024-01012-w

Figure Lengend Snippet: A Schematic of the wild-type LASV genome used to create the recombinant control Lassa virus (rLASV-WT; top) and the locations of attenuating changes in rLASV/IGR-CD (bottom). B Vero cells or C A549 cells were exposed to rLASV-WT or rLASV/IGR-CD at the indicated MOIs, and supernatants were collected at the indicated time points. Virus titers were measured by plaque assay. D Representative images showing the morphology of plaques caused by rLASV-WT and rLASV/IGR-CD in Vero E6 cells. E Vero cells or F A549 cells were exposed to rLASV-WT or rLASV/IGR-CD at the indicated MOIs, and supernatants were collected at the indicated time points. Viral RNA levels were measured by RT-qPCR. n = 3, error bars represent standard deviation. * p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.00005. CD codon-deoptimized; GCE genome copy equivalents; GP glycoprotein gene; GP CD codon-deoptimized glycoprotein gene; IGR intergenic region; L large segment; L large protein gene; LASV Lassa virus; MOI multiplicity of infection; NP nucleoprotein gene; PFU plaque-forming units; rLASV recombinant LASV; RT-qPCR real-time reverse transcription polymerase chain reaction; S small segment; WT wild-type; Z zinc-binding protein gene.

Article Snippet: The sources and growing conditions of human adenocarcinoma alveolar basal epithelial A549, human embryonic kidney epithelial HEK293T/17, and grivet ( Chlorocebus aethiops (Linnaeus, 1758)) kidney epithelial Vero (CCL-81) and Vero E6 (BEI Resources, Manassas, VA, USA; #BR596) cells were described previously , .

Techniques: Recombinant, Control, Virus, Plaque Assay, Quantitative RT-PCR, Standard Deviation, Infection, Reverse Transcription, Polymerase Chain Reaction, Binding Assay

Journal: NPJ Vaccines

Article Title: A Lassa virus live attenuated vaccine candidate that is safe and efficacious in guinea pigs

doi: 10.1038/s41541-024-01012-w

Figure Lengend Snippet: A Vero cells or A549 cells were exposed to rLASV-WT or rLASV/IGR-CD and cell lysates were collected at the indicated time points. Western blots were performed on cell lysates with antibodies to LASV NP, LASV GP, or actin beta (loading control). See Supplementary Fig. for uncropped blots. B Resin blocks containing Vero cells infected with rLASV-WT or rLASV/IGR-CD were sectioned and scanned by a transmission electron microscope. Samples contained small quantities of LASV particles measuring 80–140 nm in diameter that were budding from the plasma membranes of infected cells and extracellular mature particles. Shown are two representative images of mature rLASV-WT and rLASV/IGR-CD virus particles in extracellular space of infected Vero cells. CD codon-deoptimized; GP glycoprotein; IGR intergenic region; LASV Lassa virus; MOI multiplicity of infection; NP nucleoprotein; rLASV recombinant LASV; WT wild-type.

Article Snippet: The sources and growing conditions of human adenocarcinoma alveolar basal epithelial A549, human embryonic kidney epithelial HEK293T/17, and grivet ( Chlorocebus aethiops (Linnaeus, 1758)) kidney epithelial Vero (CCL-81) and Vero E6 (BEI Resources, Manassas, VA, USA; #BR596) cells were described previously , .

Techniques: Western Blot, Control, Infection, Transmission Assay, Microscopy, Clinical Proteomics, Virus, Recombinant

Journal: NPJ Vaccines

Article Title: A Lassa virus live attenuated vaccine candidate that is safe and efficacious in guinea pigs

doi: 10.1038/s41541-024-01012-w

Figure Lengend Snippet: Assessment of rLASV/IGR-CD stability during serial passage in cultured Vero cells

Article Snippet: The sources and growing conditions of human adenocarcinoma alveolar basal epithelial A549, human embryonic kidney epithelial HEK293T/17, and grivet ( Chlorocebus aethiops (Linnaeus, 1758)) kidney epithelial Vero (CCL-81) and Vero E6 (BEI Resources, Manassas, VA, USA; #BR596) cells were described previously , .

Techniques: Cell Culture, Residue

Journal: iScience

Article Title: Host and microbiome features of secondary infections in lethal covid-19

doi: 10.1016/j.isci.2022.104926

Figure Lengend Snippet:

Article Snippet: Vero CCL-81 cells , European Collection of Authenticated Cell Cultures , ECACC 84113001.

Techniques: Virus, Recombinant, Reverse Transcription, One Step RT-PCR, SYBR Green Assay, Gel Extraction, Western Blot, Software, Next-Generation Sequencing, Chocolate